![]() Viral RNA was extracted from stored plasma using the UltrasSens Viral Isolation kit (Qiagen), converted to cDNA using Superscript III Reverse Transcriptase (Invitrogen), and screened for HCV by quantitative polymerase chain reaction (PCR) designed to detect the HCV 5′UTR. Minority race/ethnicity was defined as self-identified African-American race or Hispanic ethnicity. ![]() Behavioral and sociodemographic data were collected via computer-assisted self-interview. Plasma was collected and syphilis, chlamydia, and gonorrhea testing performed for all participants. Recent infection was determined by one of four ways: nucleic acid amplification test with negative serology, detuned assay (Low Sensitivity Vitros ECi, Ortho Clinical Diagnostics, Rochester, NY), provider verification, or negative HIV testing within the past year. Clients were eligible if they were recently HIV-infected (<1 year of infection) or newly HIV-diagnosed, at least 18 years of age, male, and reported sex with a male partner in the past year. Our aims were to characterize the prevalence of and risk factors for HCV co-infection and patterns of HIV and HCV co-transmission and drug resistance mutations (DRMs) in a cohort of newly HIV-infected or HIV-diagnosed Los Angeles MSM.īetween February 2009 and May 2012 we enrolled participants from a community-based organization providing sexual health services to the gay community in LAC: The Los Angeles LGBT Center. has not been defined, despite LAC being the second largest epicenter for AIDS cases nationally, with high rates of non-injection drug use and high-risk sexual practices. The prevalence of HCV co-infection in HIV-infected MSM in Los Angeles County (LAC) in the U.S. ![]() Prevalence estimates for HCV co-infection in HIV-infected MSM have ranged from 6 to 15.7 %, with limited geographic characterization. Whereas the primary route of HCV transmission remains injection drug use (IDU), over recent years there has been increasing evidence of sexual transmission among HIV-infected men who have sex with men (MSM), likely driven by mucosal risk factors, including unprotected and traumatic sexual practices in the context of multiple partners, non-injection drug use, and sexually transmitted infections. Hepatitis C virus (HCV) co-infection is common amongst HIV-infected persons, affecting an estimated 4 to 5 million persons worldwide, and is associated with increased morbidity and mortality. ![]()
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